Fig. 1: Expression of HER2 in GBM samples and tumor cell lines.

A Representative images of immunohistochemical analysis showing HER2 levels in tumor and adjacent tissue from patients with GBM. After washing, the secondary antibody was incubated and visualized with 3,3′-diaminobenzidine. Light yellow indicates low expression, and deep yellow indicates high expression. GBM, glioblastoma. Scale bar = 50 and 100 μm. B Surface expression of HER2 on tumor cell lines (U118MG, U251, U87MG, HepG2, MKN-45, SK-OV-3, and HOS). HER2 expression on the cell surface was verified using flow cytometry. Numbers indicate the percentage of HER2+ cells, and 10,000 events were acquired for each analysis.