Fig. 2: Construction of 3rd-generation CAR-T cells targeting HER2.

A Schematic diagram of the HER2 CAR-T transgene. B. HER2, EpCAM, and MSLN expression was measured using flow cytometry. CAR-T cells were incubated with biotinylated anti-HER2, anti-MSLN, and anti-EpCAM, followed by incubation with APC streptavidin (1:1000). Data (B) is shown as mean ± SD of triplicates and is representative of three independent experiments.