Fig. 3: p.E300Gfs*7 mutation leads to loss of expression of ARPC1B. | Genes & Immunity

Fig. 3: p.E300Gfs*7 mutation leads to loss of expression of ARPC1B.

From: Molecular analysis and immunological characterization of a founder mutation causing ARPC1B deficiency

Fig. 3

A RT-qPCR results showing the ARPC1B mRNA levels for HEK293T cells transfected with empty vector (EV), wild-type (WT) or mutant (M) plasmids or non-transfected (NT) cells. GUS was used as a control for gene expression. Error bars represent SD for three technical replicates. Graph is representative of three independent experiments. Western blots of lysates from the transfected HEK293T cells shown in A showing expression of (B) ARPC1B and (C) His tag. α-tubulin was used as a loading control. Observed molecular weight of ARPC1B is 38 kDa. Each western blot is representative of three individual experiments.

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