Fig. 4: Effects of ZP2 PV on intracellular trafficking of ZP proteins in CHO cells.
From: ZP2 pathogenic variants cause in vitro fertilization failure and female infertility
a CHO cells transfected with ZP2ZsGreen or mutZP2ZsGreen (c.1691_1694dup [p.C566Wfs*5]) expression vectors encoding full-length or truncated proteins. Cells were fixed and imaged under confocal laser scanning microscopy (1000×). Higher-magnification inserts are images of aggregates with protein lacking C-terminal domains. b Flow cytometry analysis of ZP2ZsGreen or mutZP2ZsGreen fusion proteins in CHO cells. All values are means ± SD from six independent experiments. P = 0.00006 versus ZP2 wild type (WT). c ZP1, ZP3mcherry, or ZP4mcherry expression vectors were cotransfected with ZP2ZsGreen or mutZP2ZsGreen vectors into CHO cells. Cells were fixed and imaged under confocal laser scanning microscopy (1000×). ZP1 protein was imaged by immunofluorescent staining using anti-ZP1 antibody. d Cell lysates were immunoprecipitated with anti-ZP1 antibody. Resultant protein samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by immunoblot using antibodies against ZP1 and ZP2. The scale bars in (a) and (c) represent 10 μm
