Table 1 Summary of NGS-predicted RH alleles, known Rh serology, and DNA variants in WHO reference samples

*Sample*	*Rh serology* ^a	*ISBT alleles* ^a	*NGS-predicted antigens* ^b	*NGS-based alleles* ^b	*Relevant variation* ^c
RBC1	D+	RHD01; RHD01	D+	RHD01; RHD01
	C+c+	RHCEC; RHCEc	C+c+	RHCEC; RHCEc	Het. RHCECE-D(2)-CE* hybrid allele
	E+e+	RHCEcE; RHCEe	E+e+	RHCE03; RHCE01.01	Het. missense variant (c.676G>C); Het. missense variant (c.48G>C)
RBC4	D+	RHD01; RHD01	D+	RHD01; RHD01
	C+c−	RHCEC; RHCEC	C+c−	RHCEC; RHCEc	Hom. RHCECE-D(2)-CE* allele
	E−e+	RHCEe; RHCEe	E−e+	RHCEe; RHCE01.01	Het. missense variant (c.48G>C)
RBC5	D−	RHD01N; RHD01N	D−	RHD01N; RHD01N	Hom. RHD deletion
	C−c+	RHCEc; RHCEc	C−c+	RHCEc; RHCEc
	E−e+	RHCEe; RHCEe	E−e+	RHCEe; RHCEe
RBC12	D−	RHD04N.01 (RHDΨ)*	D−	RHD04N.01; RHD01N	Hemi. variants (37-bp insertion, c.807T>G)^d; Hemi. RHD deletion
	C−c+, V+ VS+	RHCEc; RHCEc	C−c+	RHCEc; (RHCE01.20.02)	Het. missense (c.48G>C); Het. missense (c.733C>G)
	E−e+, V+ VS+	RHCEe; RHCEe	E−e+	RHCEe; (RHCE01.20.02)	Het. missense (c.48G>C); Het. missense (c.733C>G)

^aRh serology and ISBT alleles shown are those previously reported in Boyle et al. (2013) or ISBT v.2.0 110914. ISBT allele names are adapted to avoid assuming phase between C, c and E, e indicative variants
^bNGS-based predicted antigen expression and NGS-based alleles are predicted based on detected variants shown in the “Relevant variation” column
^cComplementary DNA (cDNA) positions are relative to NM_016124.3 for RHD and NM_020485.4 for RHCE
^dIn RBC12, variants under “Relevant variation” co-occurred with 4 other variants that define the RHD*04N.01
ISBT International Society of Blood Transfusions, NGS next-generation sequencing, WHO World Health Organization

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