Fig. 4: Lipase H activity in HeLa cells transfected with wild-type (WT) lipase H and either wild-type or variant C3ORF52 was measured as detailed in Supplementary Materials and Methods.
From: Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis
Fluorescence emission at 535 nm, reflecting lipase H activity, was monitored every 120 seconds for 2 hours, immediately upon adding the PED-A1 substrate. Fluorescence data are expressed as arbitrary units (a.u.). Data represent the mean values ± standard deviation (SD) of four independent experiments done in duplicates (time series regression; *p < 0.05). Background values recorded from extracts of HeLa cells transfected with a pcDNA3.1 empty vector were subtracted from the experimental readings.
