Fig. 2: Characterization of orthologous genetic variants in mice.
From: Rare variants in KDR, encoding VEGF Receptor 2, are associated with tetralogy of Fallot
(a) Hemtoxylin and eosin (H&E) stained sections of KdrG347W/+, KdrG347W/G347W, and compound heterozygotes KdrG347W/G537R mice. Scale bar is 0.5 cm. (b) Whole mount images E10.0 Kdr+/+ and KdrG535R/G535R embryos. Normal vasculature (black arrows) as seen in Kdr+/+ are not visible in KdrG535R/G535R embryos, indicating impaired endothelial development. Scale bar is 1 mm. (c) H&E stained sections of the developing heart of the Kdr+/+ and KdrG535R/G535R embryos from (b), showing enlarged pericardial cavity in KdrG535R/G535R embryos (asterisk). Scale bar is 250 um. (d) Anti-VEGFR2 stained single optical sections of endocardial cells from Kdr+/+ and KdrG535R/G535R embryos. VEGFR2 is labeled green, WGA (cell membranes) is labeled red, and nuclei are labeled blue. Yellow arrowheads denote colocalization at the membrane of VEGFR2 and WGA and white arrowheads denote cytoplasmic VEGFR2. Scale bar is given in the figure. h heart, he head, pa pharyngeal arches, WGA wheat germ agglutinin.
