Fig. 3: Phosphorylation assay.
From: Rare variants in KDR, encoding VEGF Receptor 2, are associated with tetralogy of Fallot
(a,b) Western blot analysis with VEGFR2 antibody or p-Y1175 antibody respectively in HEK 293T cells. The lanes contain (from left to right) HEK293T cells transfected with an empty vector (pcDNA), wild-type KDR (WT), KDR-p.(Gly345Trp) alone, KDR-p.(Gly537Arg) alone, KDR-p.(Gly345Trp) with WT, KDR-p.(Gly537Arg) with WT and KDR-p.(Gly345Trp) together with KDR-p.(Gly537Arg). (a',b’) Quantification of western blots in (a) and (b). In (a’): n = 5 for each condition, except WT + KDR-p.(Gly345Trp) and WT + KDR-p.(Gly537Arg) (both n = 3). In (b'): n = 3 for each condition. *Standard two-sided t-test p < 0.05 compared to WT; **p < 0.01 compared to WT. (c) Western blot analysis with VEGFR2 antibody on yolk sac cells of wild-type and knock-in mice. Each lane represents one experiment. Gel has been edited to group genotypes; the unedited image can be found as Supplemental figure S1A. (d) Quantifications western blot analysis with p-Y1175 antibody in HEK 293T cells. The lanes contain (from left to right) HEK293T cells transfected with an empty vector (pcDNA), wild-type KDR (WT), KDR-p.(Val219Ala) alone, KDR-p.(Thr442Met) alone, KDR-p.(Val219Ala) with WT, KDR-p.(Thr442Met) with WT and KDR-p.(Gly345Trp) together with KDR-p.(Gly537Arg). n = 4 for each condition. *Standard two-sided t-test p < 0.05 compared to WT; **p < 0.01 compared to WT. Representative western blot can be found as Supplemental figure S1B.
