Fig. 2

Splice variants (a) and PCR products of dsx (b) using RNA extracted from the thorax of teneral individuals. a The long transcript that includes region 4 and the short transcripts that lack this region were detected using 25 teneral individuals. The red box indicates the conserved functional domain (DM1). The blue boxes indicate the open reading frames. The red arrows indicate primers used in PCR amplification and sequencing. The numbers above the boxes are region identifiers. b PCR products of dsx of a teneral male (M), an andromorph (A), and a gynomorph (G) are shown. The long transcript was specifically expressed by andromorphic (n = 3) and gynomorphic females (n = 11), and the short transcripts were expressed by males (n = 8) and andromorphic females (n = 3). Short transcripts were detected in some of the gynomorphs when 40 cycles of PCR amplification were performed. The numbers on the right-hand side of the figure show the combination of putative exons corresponding to each band