Fig. 1

Map showing current range of Chrysemys picta with sampling sites for this study. Blue lines indicate approximate boundaries for traditional subspecies, with variation in morphology among species shown for three representative individuals (C. picta picta from Orange County, NY; C. picta marginata from Walworth County, WI; C. picta bellii from Buffalo County, WI). Black dots indicate sampling sites for the range-wide mitochondrial DNA dataset, with regional groupings delineated by dashed lines and labeled with numbers. Red diamonds show locations for populations-level datasets. Four hypothesized refugial locations are shown: one on the East Coast (EC), one on the Gulf Coast (GC), one in the Southwestern US/Mexico (SW), and one broad southeastern refuge encompassing the East and Gulf Coasts (SE)