Table 1 Pairwise F_ST and Φ_ST values estimating differentiation across contemporary and putative historical biogeographical barriers between the populations of grey shrike-thrush inhabiting the regions either side of each barrier

Regions compared	Barrier	Nuclear intron Φ_ST						Length-variable marker F_ST	ND2 Φ_ST
Regions compared	Barrier	AB4	DRD4	GAPDH	MUSK-I4	RI2	TGFb2	Length-variable marker F_ST	ND2 Φ_ST
East-Australia V Tasmania	Bass Strait	0.49 (<0.001)	0.57 (<0.001)	0.48 (<0.001)	0.09 (0.009)	0.19 (0.027)	0.29 (<0.001)	0.34 (<0.001)	0.87 (<0.001)
East-Australia V Cape York Peninsula	Torresian	0.51 (<0.001)	0.00 (0.387)	0.15 (0.288)	0.93 (<0.001)	0.06 (0.153)	0.10 (<0.001)	0.07 (<0.001)	0.66 (<0.001)
East-Australia V north-west	Carpentarian	0.48 (<0.001)	0.25 (<0.001)	0.00 (0.315)	0.93 (<0.001)	0.04 (0.207)	0.10 (<0.001)	0.09 (<0.001)	0.91 (<0.001)
East-Australia V south-west/central	Eyrean	0.50 (<0.001)	0.20 (<0.001)	0.169 (<0.001)	0.85 (<0.001)	0.44 (<0.001)	0.18 (<0.001)	0.07 (<0.001)	0.91 (<0.001)
Cape York Peninsula V PNG	Torres Strait	0.14 (0.216)	0.03 (0.126)	−0.27 (0.991)	0.00 (0.423)	0.05 (0.378)	0.10 (0.091)	NA	0.19 (<0.001)
Cape York Peninsula V north-west	Carpentarian	0.24 (0.027)	0.36 (0.009)	0.18 (0.108)	0.68 (<0.001)	−0.08 (0.991)	0.10 (0.054)	0.05 (<0.001)	0.92 (<0.001)
North-west V south-west/central	Canning	0.09 (0.198)	0.369 (<0.001)	−0.06 (0.68)	0.94 (<0.001)	0.67 (<0.001)	0.46 (<0.001)	0.08 (<0.001)	0.94 (<0.001)

Significant (P-value < 0.011) estimates are in bold. Length-variable marker data were not available for the Papua New Guinean (PNG) range of the species, so differentiation across the Torres Strait could not be estimated using this dataset (NA)

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