Table 2 Loci and primer sequences used for sexing and copy number qPCR.
Gene | Purpose | Forward primer (5′–3′) Reverse primer (5′–3′) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
CTNNB1 | Sexing PCR | TAAGGTCCTGGGCATGTCCT ATGGCTTGGAATGAGGTGGG | 60.25 60.03 | 1289; 1488 |
EEF1A1 | Copy number qPCR | TGTGCTGTCCTTATTGTTGCTG ATGTGCCGACTTCTTTGACAATCTC | 59.45 61.82 | 199 |
TANC2 | Copy number qPCR | TGGCAAAATGGACAACACAACC CCGAAAGGGAGACAGGAACTAC | 60.42 60.09 | 161 |
GH1 | Copy number qPCR | GCAGGGACCAGTAGACCTTC TGTTGCGAGCTGATGGAGAT | 59.46 59.46 | 153 |
SCN1A | Copy number qPCR | TGAATGCCCTTCTTGGAGCA ACTTCAAACATGTTGCCAGTTGT | 59.59 59.80 | 151 |
SCN2A | Copy number qPCR | TCGCTGGCAAGTTCTACCAC AGAGGTAGCCAAGTCCCACA | 60.32 60.18 | 165 |
SCN3A | Copy number qPCR | GCCCTTGTTGGAGCAATTCC TTCCATCGGGCTTGCTGATT | 59.75 60.03 | 212 |
SCN4A | Copy number qPCR | AATGCTCTTTTGGGGGCCAT TGACCCAACGGACATCAGTG | 60.25 59.97 | 223 |
SCN5A | Copy number qPCR | AACCCATCACCACTACGCTG CATCATGCAGGCAATGAGGC | 60.04 59.97 | 182 |
SCN8A | Copy number qPCR | ACAGGGTCCGTTTAGCAAGG TCGGCTCGCTGTTTCTCTTT | 59.96 59.97 | 166 |
SCN9A | Copy number qPCR | CCCCACAAGGAGCAATAGCA ACACTGCTCTGCATCTTCCC | 60.03 60.04 | 210 |
SCN10A | Copy number qPCR | TCGAAAATGGCCTCGCTTCT GTGGTCCAAGGCCATGAAGA | 60.04 59.96 | 175 |
SCN11A | Copy number qPCR | CAACAGTACGTGCCAGTTGC ACCAGGACCAGCATGAACAG | 60.04 59.96 | 159 |
