Table 4 Hardy–Weinberg analysis of alleles of the SCN4A locus, encoding the DIV p-loop region of Nav1.4.

From: Sex linkage of the skeletal muscle sodium channel gene (SCN4A) explains apparent deviations from Hardy–Weinberg equilibrium of tetrodotoxin-resistance alleles in garter snakes (Thamnophis sirtalis)

 

Autosomal

Z-linked

Population

n homozygotes

n heterozygotes

HO

HE

HWE

HO

HE

HWE & EAF

Cook Creek

8

2

0.125

0.219

0.190

0.200

0.180

1.000

Potters Slough

5

3

0.158

0.481

0.001

0.375

0.430

1.000

Warrenton

9

4

0.174

0.360

0.012

0.308

0.382

0.257

Hebo

3

3

0.200

0.604

0.001

0.500

0.611

0.708

Benton

0

3

0.150

0.524

0.000

1.000

0.611

0.347

Ten Mile

3

7

0.438

0.596

0.044

0.700

0.535

0.451

Tahkenitch

10

3

0.103

0.212

0.032

0.231

0.311

0.182

Elk River

3

0

0.000

0.360

0.000

NA

NA

0.324

Willits

6

0

0.000

0.180

0.053

0.000

0.278

0.100

Hopland

17

0

1.000

NA

NA

1.00

NA

NA

Knoxville

7

2

0.111

0.278

0.038

0.222

0.198

0.655

Russian River

4

0

0.000

0.444

0.005

0.000

0.375

0.002*

Ledson Marsh

6

1

0.083

0.622

0.000

0.143

0.459

0.033*

  1. First, observed heterozygosity (HO), expected heterozygosity (HE), and p values for tests of deviation from HWE are shown when SCN4A is assumed to be autosomal. Next, SCN4A is assumed to be Z-linked and we test jointly for deviations from HWE and EAF. Here, HO and HE are calculated for only males. Monomorphic populations, for which HWE cannot be estimated, were excluded from this analysis and HWE was not calculated for the Elk River population due to the low sample size.
  2. Significant deviations from HWE and EAF are indicated with asterisks.
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