Table 2 Fifteen microsatellite markers developed to genotype Drosophila melanogaster in this study.
Locus | Chr. | Position | Repeat motif | Allele number | Size range (bp) | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|---|---|---|---|---|
S04 | 2L | 2,932,573 | AGC | 6 | 136–149 | CTTCGCCTTTCCAGCTTTGT | GTCGTAGTTGTGTCGCTGTC |
S09 | 2R | 23,181,060 | AGC | 10 | 135–175 | GGGCTTCCTCATCCGGAATT | GCCCACCTCGCTGATAATTG |
S10 | 2R | 12,896,467 | AGCC | 21 | 141–229 | GTCCTTAGTCGAACCGCTCT | CACGAGCGACCATTGCATTT |
S14 | 2R | 13,082,536 | AGC | 9 | 159–194 | TTGCTTTGTCTGATTGCGGA | CGAATCTGGCAACTGTGGAG |
S17 | 2L | 3,419,414 | AGC | 8 | 183–206 | TCCGTTCCCATTCTTCTCGG | GCTCCGGCTGCAACTTAATT |
S18 | 3L | 12,644,547 | AGC | 8 | 181–214 | CAAGGCAGAGCATCACCATC | CAAACTCACTTCCTGCGCTG |
S28 | 3R | 15,812,776 | ACT | 3 | 223–235 | ATCCACGTCCCACCAGAAG | AAACGCACAACAACGCCATA |
S39 | 2L | 21,941,431 | ATCCG | 9 | 265–304 | AAACGCATTTGCCCACCTTT | GGCACCGAACATCAATCTCC |
S41 | 2L | 9,658,593 | AGC | 11 | 290–325 | AGTTAACAAGTGACACGCCC | ATCTCGAATCGCCTTGGCTA |
S42 | 2L | 16,449,207 | ACC | 6 | 287–305 | TATTTAAGGGCATGGCGGGT | GCCACGAGGAATTGGACATC |
S44 | 2R | 11,206,856 | AGC | 8 | 312–337 | GAGGTCGGTGAAAGGAGTGG | CGATCATCATTCTGCCGCAA |
S47 | 2R | 13,921,847 | AGCC | 11 | 307–357 | GGTGCGCTCCTTAATGTCAG | ATGCATAAAGGTCCGGCAAG |
S48 | 3R | 5,290,483 | AGG | 4 | 324–336 | GCTTCCCTCTCCAATTCTGC | ATGGATGGGCACTAACGGAA |
S52 | 3L | 13,589,683 | AGC | 7 | 350–367 | GCCTTGCCAAGTTTCCAGTT | AAGCACACCATATCGCAGAC |
S55 | 3L | 8,663,943 | ATC | 7 | 343–359 | GTTGTTGAAAGCGATTGCCC | CTTGTTGACGCGGCATCTAA |
