Fig. 7: The CYS activity analysis of BmorCYS/SfruCYS1/SfruCYS2. | Heredity

Fig. 7: The CYS activity analysis of BmorCYS/SfruCYS1/SfruCYS2.

From: Horizontally acquired cysteine synthase genes undergo functional divergence in lepidopteran herbivores

Fig. 7: The CYS activity analysis of BmorCYS/SfruCYS1/SfruCYS2.The alternative text for this image may have been generated using AI.

A Michaelis–Menten analysis of recombinant BmorCYS/SfruCYS1/SfruCYS2. Various concentrations of OAS and sodium sulfide were used to determine the kinetic parameters of the recombinant CYSs. B pH profile of recombinant BmorCYS/SfruCYS1/SfruCYS2. The pH–activity relationship was determined using 10 mM OAS and 5 mM sodium sulfide as the substrate over a pH range of 3–13. Recombinant CYSs (1 µg) were incubated for 15 min at 30 °C. Cysteine was quantified by measuring the absorbance at 560 nm. These experiments were repeated three independent times.

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