Fig. 4: D. gunungcola SK alleles linked to ebony on Muller element E have varied effects on thorax, leg, and wing pigmentation divergence.
A Images highlighting pigmentation and chromosome differences between species and the Muller Element E introgression line. The white dashed box highlights the scutellar region of the thorax used to quantify thorax pigmentation in (B) (see Methods). Dashed black lines highlight regions of the wing and leg used to quantify wing spot and leg pigmentation in (B) (see Methods). B Quantification of thorax pigmentation differences between species and the Muller element E introgression line (One-way ANOVA F3,35 = 33.9; P = 1.84 × 10−10; groups not sharing the same letter are significantly different based on post-hoc Tukey HSD at P < 0.00001). Quantification of wing spot pigmentation differences between species and the Muller element E introgression line (One-way ANOVA F2,88 = 78.6; P < 2.0 × 10−16; groups not sharing the same letter are significantly different based on post-hoc Tukey HSD at P = 0.02) (Reprinted from Massey et al. (2020a), Copyright (2020), with permission from John Wiley and Sons). Quantification of leg pigmentation differences between species and the Muller element E introgression line. (One-way ANOVA F3,33 = 481; P < 2.0 × 10−16; groups not sharing the same letter are significantly different based on post-hoc Tukey HSD at P < 5 × 10−7). Transparent circles represent individual replicates for (B).
