Fig. 1: Pedigree depicting breeding design and line plot showing experimental temperatures.

The pedigree (A) is limited to experimental individuals with data on all four investigated traits (n = 538; 32 families in generation 2) and shows relationship ties via their reassigned parents (generation 1) up to their reconstructed grandparents (generation 0). Offspring are connected to their mother by turquoise lines and to their father by violet lines. The line plot (B) shows the average daily water temperatures for each thermal regime, which are differentiated by blue (ambient; cold), yellow (optimum), and red lines (warm). The phenotyping period is indicated by the grey vertical bar.