Fig. 5: Comparative C-banding and FISH for repeat location between the L1 and B chromosomes of A. flavolineata.

The L1 autosome showed, like the remaining A chromosomes, a large pericentromeric C-band including the pericentromeric region and the short arm. The FISH analysis for seven satDNA families and the U2 snDNA repeat showed pericentromeric and telomeric locations on the B chromosome whereas they were located on the pericentromeric region and the short arm of L1 (AflSat01, AflSat02, AflSat03, the pericentromeric region (AflSat46), interstitial (AflSat07), interstitial region and the short arm (AflSat25 and AflSat52). satDNAs thus might suggest that B originates from the proximal third of L1, including the interstitial region containing several satDNAs. However, the U2 snDNA is located on an L1 region outside the former proximal region, so the presence of U2 on B is not explained by a single rearrangement event.