Table 1 Correlation between the distribution of nucleotide composition and RSCU (codon with G/A at 3rd position) in the mitogenomic protein-coding genes and habitat (freshwater/euryhaline and marine) of clupeoid fishes.

From: Comparative mitogenomics of Clupeoid fish provides insights into the adaptive evolution of mitochondrial oxidative phosphorylation (OXPHOS) genes and codon usage in the heterogeneous habitats

Correlation between nucleotide composition in the protein-coding genes and habitat (freshwater/euryhaline and marine) of clupeoid fishes

Nucleotide position

T-1

C-1

A-1

G-1

T-2

C-2

A-2

G-2

T-3

C-3

A-3

G-3

All species except Engraulidae

Point-Biserial Correlation

Correlation Coefficient (r)a

−0.04

0.08

0.43

−0.56

−0.30

0.22

0.31

−0.29

−0.18

−0.04

0.53

−0.57

p value

>0.05

>0.05

<0.05

<0.01

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

<0.03

<0.01

Chi square test (Crosstab analysis)

X-squared (χ2)b

2.4

6.0

13.6

13.5

3.4

4.6

13.5

8.2

0.2

6.2

51.1

55.0

p value

>0.05

>0.05

<0.001

<0.001

>0.05

>0.05

<0.001

0.04

>0.05

>0.05

<0.001

<0.001

All species in Engraulidae

Point-Biserial Correlation

Correlation Coefficient (r)a

0.51

−0.31

0.61

−0.80

0.02

−0.32

0.38

−0.17

0.21

−0.51

0.83

−0.89

p value

>0.05

>0.05

<0.05

<0.01

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

<0.01

<0.001

Chi square test (Crosstab analysis)

X-squared (χ2)b

2.7

3.8

5.9

4.3

5.0

1.3

0.5

2.5

1.7

1.7

8.2

7.8

p value

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

<0.05

<0.05

Correlation between RSCU in the protein-coding genes (codon with G/A at 3rd position) and habitat (freshwater/euryhaline and marine) of clupeoid fishes

All species except Engraulidae

Codon with G/A at 3rd position

Leu

Met

Val

Ser

Pro

Thr

Ala

Gln

Lys

Glu

Trp

Gly

TTA

TTG

CTA

CTG

ATA

ATG

GTA

GTG

TCA

TCG

CCA

CCG

ACA

ACG

GCA

GCG

CAA

CAG

AAA

AAG

GAA

GAG

TGA

TGG

GGA

GGG

Point-Biserial Correlation

Correlation Coefficient (r)c

0.05

−0.58

0.70

−0.51

0.48

−0.48

0.55

−0.35

0.45

−0.67

0.59

−0.75

0.57

−0.55

0.37

−0.62

0.65

−0.65

0.64

−0.64

0.64

−0.64

0.54

−0.54

0.53

−0.48

p value

>0.01

<0.001

<0.0001

<0.01

<0.01

<0.01

<0.01

>0.01

<0.01

<0.001

<0.001

<0.0001

<0.01

<0.01

>0.01

>0.01

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.01

<0.01

<0.01

<0.01

Chi square test (Crosstab analysis)

X-squared (χ2)d

11.2

28.2

25.6

28.6

34.9

34.5

26.3

19.4

25.8

33.3

18.5

24.3

27.8

33.0

12.8

30.7

32.6

37.0

39.9

39.5

24.6

26.0

22.3

21.3

27.0

22.6

p value

<0.01

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

All species in Engraulidae

Codon with G/A at 3rd position

Leu

Met

Val

Ser

Pro

Thr

Ala

Gln

Lys

Glu

Trp

Gly

TTA

TTG

CTA

CTG

ATA

ATG

GTA

GTG

TCA

TCG

CCA

CCG

ACA

ACG

GCA

GCG

CAA

CAG

AAA

AAG

GAA

GAG

TGA

TGG

GGA

GGG

Point-Biserial Correlation

Correlation Coefficient (r)c

0.32

−0.76

0.83

−0.70

0.68

−0.68

0.82

−0.46

0.70

−0.85

0.73

−0.86

0.78

−0.68

0.56

−0.80

0.83

−0.83

0.82

−0.82

0.80

−0.80

0.76

−0.76

0.70

−0.66

p value

>0.05

<0.001

<0.001

<0.01

<0.01

<0.01

<0.001

>0.05

<0.01

<0.001

<0.001

<0.001

<0.001

<0.01

<0.05

<0.01

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.01

<0.001

Chi-square test (Crosstab analysis)

X-squared (χ2)d

1.2

3.0

5.4

1.8

3.5

2.4

3.2

2.2

1.3

4.4

7.9

6.5

7.8

8.3

8.3

3.8

7.3

8.3

9.9

11.3

6.0

6.5

6.2

8.7

6.0

8

p value

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

>0.05

<0.05

>0.05

<0.05

<0.05

<0.05

>0.05

<0.05

<0.05

<0.05

<0.05

>0.05

>0.05

>0.05

<0.05

>0.05

<0.05

  1. aCalculated using Point-Biserial correlation analysis of the percentage of nucleotides in the concatenated protein-coding genes of clupeoid fishes with its habitat (freshwater/euryhaline and marine).
  2. bChi-square test in Crosstab analysis for association of the percentage of nucleotides in the concatenated protein-coding genes of clupeoid fishes with its habitat (freshwater/euryhaline and marine).
  3. cCalculated using Point-Biserial correlation analysis of RSCU (codon with G/A at 3rd position) in the concatenated protein-coding genes of clupeoid fishes with its habitat (freshwater/euryhaline and marine).
  4. dChi-square test in Crosstab analysis for association of the RSCU (codon with G/A at 3rd position) in the concatenated protein-coding genes of clupeoid fishes with its habitat (freshwater/euryhaline and marine).