Fig. 6: Differential regulation of GLH17, an example of a gene that is strongly differentially spliced and also differentially expressed between ecotypes; GLH17 functions in lateral root emergence.
From: Gene expression and alternative splicing contribute to adaptive divergence of ecotypes
A Structures of two alternative isoforms of GLH17, which constitute a case of exon skipping. The “skipping” isoform is above the “inclusion” isoform, with the skipped exon marked by a red box; isoforms are marked for whether they are more prevalent in the dune (yellow triangle) or non-dune (green circle) ecotype. Dark blue blocks represent coding exons and light blue represents exons in untranslated regions (UTRs). The alternatively spliced (skipped) exon is within the 5′ UTR. B GLH17 gene-level normalized expression differences between dune (D) and non-dune (N-d) ecotypes (log2 fold change = −4.3e-05, FDR < 0.05) and differences in alternative splicing (ΔPSI = −0.644, FDR « 0.001) for the exon skipping event. “Percent Spliced In” (PSI) refers to the proportion of reads supporting the inclusion isoform. Points represent individual plants.
