Table 2 Second experiment: Linear mixed model on embryo hatching time, hatchling length, and yolk sac volume at hatching when predicted by treatment (exposure to Aeromonas salmonicida; fixed effect) and dam, sire, dam x sire, and their interaction with treatment (random factors).

Effects	Hatching time				Hatchling length				Larval length 130dpf				Yolk sac volume 130dpf
Fixed effects	Coefficients	DF	t	p	Coefficients	DF	t	p	Coefficients	DF	t	p	Coefficients	DF	t	p
Intercept	93.39 ± 0.43	9.44	218.3	<0.001	7.07 ± 0.31	1836	23.0	<0.001	21.18 ± 0.40	1154	52.5	<0.001	−27.20 ± 4.16	1452	−6.53	<0.001
Treatment	−0.33 ± 0.10	8.45	−3.34	0.01	−0.10 ± 0.007	2997	−14.5	<0.001	−0.10 ± 0.01	2399	−11.3	<0.001	0.05 ± 0.10	2709	0.50	0.62
Hatching time					0.06 ± 0.003	2692	18.0	<0.001	−0.05 ± 0.004	2086	−12.2	<0.001	0.61 ± 0.04	1920	13.84	<0.001
Random effects^a	Variance components				Variance components				Variance components				Variance components
Dam	1.17 ± 0.67			0.08	0.025 ± 0.014			0.07	0.07 ± 0.04			0.07	4.61 ± 2.51			0.07
Sire	0.55 ± 0.23			0.01	0.007 ± 0.003			0.008	0.01 ± 0.004			0.007	0.27 ± 0.17			0.12
Dam × sire	0.38 ± 0.15			0.01	0.004 ± 0.002			0.004	0.004 ± 0.002			0.06	0.62 ± 0.25			0.015
Dam × treatment	0.07 ± 0.07			0.33
Sire× treatment	0.07 ± 0.08			0.40
Dam × sire × treatment	0.88 ± 0.17			<0.001
Residual	4.13 ± 0.11				0.158 ± 0.004				0.20 ± 0.006				24.5 ± 0.68

Hatching time was included as a further covariate when testing for effects on hatchling and larval lengths and yolk sac volume. The table gives the parameters as in Table 1 and after the stepwise removal of non-significant interaction terms. Significant p-values are highlighted in bold.
^aREML unbounded variance components ± standard error, Wald p-values.

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