Fig. 1: The ability of an LNA probe assay to measure heteroplasmy in PSHB.
From: Pervasive heteroplasmy in an invasive ambrosia beetle (Scolytinae) in southern California
Incremental mixes of H33 and H35 were prepared from pooled homoplasmic DNA (H33+ and H35+; x-axis). The abundance of the H33 (light gray) and H35 (dark gray) mitotypes relative to each other was then estimated in each mix based on the fluorescence emitted by their respective LNA probes following normalization to a single scale (see text). Validation was performed in three replicate runs (solid bars vs. striped bars vs. patterned bars, respectively). The third run was extended to include the two mitotypes in a ratio of 95:5 relative to each other. No-template controls were also run but are not plotted since their incorporation into the normalization of the change in fluorescence of each probe results in zero values.
