Fig. 5: Relative mitotype titers in six iso-female lineages of PSHB instigated from a heteroplasmic parental colony (P) and reared at 29 °C (29-B, -C, and -D) or 23 °C (23-C, -E, and -F).
From: Pervasive heteroplasmy in an invasive ambrosia beetle (Scolytinae) in southern California
Titers were estimated for each lineage from a minimum of four individuals at generations 1 and 6 (all lineages), and in lineages reared at 29 °C, also at generation 10. The abundance of H33 (light gray) and H35 (dark gray) mitotypes relative to each other was estimated using an LNA probe assay (see text). Homoplasmic H33 and H35 DNA stocks, and a 50:50 mix of the two, were used as positive controls. No-template controls were also run but are not plotted since their incorporation into the normalization of the change in fluorescence of each LNA probe results in zero values.
