Table 1 PCR primers and LNA probes for relative quantification of mitotype titers.

From: Pervasive heteroplasmy in an invasive ambrosia beetle (Scolytinae) in southern California

Primer/Probe

Oligo sequence (5′–3′)

Bases

Tm (°C)a

PSHB-HetCOI-F

TAGCTGGAGGAATCACCATATT

22

61.0

PSHB-HetCOI-R

TTGATATAGGACAGGATCTCCAC

23

61.0

H33-Probeb

HEX-TG + A + G + GTGT + T + A + ATATTTC-IABkFQc

17

63.8

H35-Probeb

FAM-AG + G + T + GT + T + G + ATATT-IABkFQc

13

64.1

  1. Primers and PrimeTime® LNA® probes were designed and synthesized by Integrated DNA Technologies, Inc.
  2. aInformation supplied by manufacturer.
  3. bNucleotides preceded by a “+” indicate an incorporated LNA monomer.
  4. cProbe quencher IABkFQ = Iowa Black FQ®.
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