Fig. 1: Overview of embryonic and sperm sampling in experiments.

A Schematic of the embryonic transcriptome fish set-up, with competition environments highlighted and data acquisition in for RNA-seq experiment. B Schematic of the male competition environments in each treatment group for sperm sRNA sequencing experiment, namely a High competition treatment (two males and one female) and a Low competition treatment (one male and two females). All males were exposed to both treatments in randomised order with half starting High followed by Low and the other half vice versa. 1 indicates first treatment and 2 indicates second treatment. Sperm samples were collected after each treatment round for sequencing. We used n = 10 males in each group resulting in a total of 40 sperm samples at the two time points. Red fish are male zebrafish, and blue fish are female zebrafish.