Fig. 4: Differential expression of mRNAs in 24 hpf half-sibling embryos sired by males exposed to High and Low competition treatments.

A Principal component analysis with treatment (Low versus High), and female ID as factors from DESeq2. B GO term analysis looking at biological processes for zebrafish using ShinyGO on all significantly differentially expressed genes (FDR cut-off was set to padj < 0.05) revealed a significant prediction to affect muscle development and differentiation. C Enhanced volcano plot of differentially expressed genes from DESeq2.