Fig. 6: Embryo ploidy of kernels produced by crossing pollen from High Loss with B chromosomes to the tetraploid.
From: Aneuploidy and ploidy variation conditioned by the B chromosome of maize
A simplified cocktail of green fluorescent probes was used to label the A chromosomes; the B-chromosome-specific probe is shown in red. A Triploid (30 A chromosomes), the expected ploidy from pollination of the egg cell by a haploid sperm (one copy of High Loss chromosome 3, arrowhead). B Tetraploid (40 A chromosomes), the result of fertilization by a diploid sperm (two copies of High Loss chromosome 3, arrowheads). C Diploid (20 A chromosomes), an unexpected result. Paternal chromosomes were lost post-fertilization as evidenced by the absence of High Loss chromosome 3 and the presence of B chromosomes. Scale bar, 5 μm.
