Fig. 2: Fluorescence in situ hybridization (FISH) of the CenR1 repeat on pachytene chromosomes of two nightingale species. | Heredity

Fig. 2: Fluorescence in situ hybridization (FISH) of the CenR1 repeat on pachytene chromosomes of two nightingale species.

From: Germline-restricted chromosome of songbirds has different centromere compared to regular chromosomes

Fig. 2: Fluorescence in situ hybridization (FISH) of the CenR1 repeat on pachytene chromosomes of two nightingale species.

Pachytene chromosomes are stained by an antibody against the lateral components of the synaptonemal complex, anti-SYCP3 antibody (blue). FISH-mapping of LmegCenR1 repeat on L. megarhynchos pachytene spreads (A1) and LlusCenR1 repeat on L. luscinia pachytene spreads (B1) (red). The centromeres on regular chromosomes (indicated by arrows) and the whole GRC (indicated by arrowheads and magnified in insets) are labeled by the CREST antibody (green) (A2, B2). CenR1 repeat signal colocalizes with the CREST signal in all chromosomes except the GRC, which show no CenR1 repeat signal (A3, B3). Scale bar = 10 µm.

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