Fig. 4: Immunofluorescence images showing the distribution of histone methylation marks and HP1 in a line carrying a B chromosome. | Heredity

Fig. 4: Immunofluorescence images showing the distribution of histone methylation marks and HP1 in a line carrying a B chromosome.

From: Chromatin state dynamics of autosomes and the B chromosome during spermatogenesis in Pseudococcus viburni

Fig. 4

A H4K20me3, (B) H3K9me3, (C) H3K27me3 in green; HP1 in red; DNA (Hoechst) in blue. All three marks are present on the B chromosome throughout meiosis even after the B chromosome has undergone changes in chromatin compaction (prometaphase I), which are visible with a less intense Hoechst staining than the other chromosomes. HP1 serves as an additional marker for the B chromosome in addition to the visible change in intensity of Hoechst staining, as HP1 is more enriched on the B chromosome than on the other chromosomes during telophase I and meiosis II. Arrowheads point to B chromosomes when visible. (P I prophase I, Mid-P I mid-prophase I, PM I prometaphase I, M/A I metaphase/anaphase I, T I telophase I, M/A II metaphase/anaphase II, T II telophase II). Scale bar: 5 µm.

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