Fig. 2: Schematic overview of experimental design in this study.
From: Maternal inheritance of primary sex ratios in the dark-winged fungus gnat Lycoriella ingenua
A Experimental design to examine heritability of primary sex ratios. After establishing isofemale lines from mass cultures, sibling crosses were set up over successive generations and offspring counted; the last flies to be counted were the F4 generation (i.e. offspring of F3 females). B For the temperature experiment flies were either held at 18 °C or moved to 25 °C or 12 °C for the duration of pupation (oogenesis). Upon emergence, females that had been subjected to different temperatures for pupation were mated and their offspring counted.
