Fig. 4: Genome wide diversity in leopards from Western Cape (WCP) and Mpumalanga (MPL) in South Africa.

A Nucleotide diversity measured with Pixy along the genome in non-overlapping windows (10 kb). B Average count of Runs of Homozygosity (ROH) of different lengths per individual in WCP and MPL. C The fraction of short < 5 Mb and long ROHs > 5 Mb of the total length of ROHs (Statistical test method, two-tailed t test). Points in the box plot are population means of per-individual proportions (error bars are 95% CI of mean). D Correlation between observed heterozygosity (HO) and proportion of the autosomal genome in ROH (FROH) in WCP and MPL (Statistical test method, Spearman correlation). E Deleterious alleles per called site (loss of function and missense mutations) measured with Ensembl VEP. The box plot represents the overall distribution of the data, and the lines in the boxes represent the mean value. F Homozygous versus heterozygous deleterious sites (n = 900), accounting for population-level difference in heterozygosity between WCP and MPL.