Table 2 Proteins identified by MALDI-TOF/TOF MS
Spot no.a | Protein name | NCBI accession no. | Protein score | Covb (%) | Peptide count | kDa/pI | Fold changec | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Theoretical | Experimental | Cs-28 °C/Cs-28 °C | Mc-28 °C/Cs-28 °C | Cs-42 °C/Cs-28 °C | Mc-42 °C/Cs-42 °C | ||||||
Photosynthesis (33) | |||||||||||
4 | RuBisCO large subunit-binding protein subunit alpha | gi|449456032 | 732 | 63% | 30 | 61.40/5.06 | 66.01/4.85 | 1.00 | 0.87 | 2.13 | 0.77 |
10 | Rubisco large subunit-binding protein subunit beta, chloroplastic | gi|449452644 | 1,120 | 59% | 29 | 64.75/5.86 | 66.05/5.33 | 1.00 | 0.40 | 2.00 | 0.37 |
15 | Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic | gi|449446650 | 586 | 63% | 22 | 48.85/5.02 | 46.50/4.81 | 1.00 | 1.01 | 1.53 | 0.72 |
21 | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic | gi|449441384 | 707 | 54% | 19 | 51.77/5.58 | 53.25/5.20 | 1.00 | 1.37 | 1.55 | 0.85 |
23 | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic | gi|449441384 | 761 | 58% | 22 | 51.77/5.58 | 54.00/5.14 | 1.00 | 2.35 | 2.37 | 0.85 |
25 | Sedoheptulose-1,7-bisphosphatase, chloroplastic | gi|229597543 | 602 | 61% | 25 | 42.08/5.96 | 41.50/5.15 | 1.00 | 0.93 | 0.62 | 0.80 |
26 | Oxygen-evolving enhancer protein 1 | gi|700193260 | 445 | 63% | 17 | 34.94/6.24 | 35.33/5.13 | 1.00 | 1.98 | 1.06 | 1.38 |
27 | Oxygen-evolving enhancer protein 1 | gi|700193260 | 600 | 56% | 16 | 34.94/6.24 | 35.75/5.24 | 1.00 | 1.94 | 0.96 | 1.29 |
28 | Oxygen-evolving enhancer protein 1 | gi|700193260 | 971 | 63% | 18 | 34.94/6.24 | 35.50/5.36 | 1.00 | 1.02 | 0.56 | 1.79 |
35 | Triosephosphate isomerase, chloroplastic | gi|449458564 | 657 | 58% | 19 | 32.72/7.01 | 30.75/5.73 | 1.00 | 0.73 | 1.54 | 0.68 |
36d | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|545698970 AFH05588 | 302 304 | 50% 54% | 77 | 20.29/6.58 18.80/5.92 | 16.00/5.41 | 1.00 | 0.74 | 0.39 | 1.37 |
37d | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|545698970 AFH05590 | 280 282 | 21% 27% | 5 5 | 20.29/6.58 18.10/5.50 | 15.50/5.23 | 1.00 | 0.47 | 0.26 | 1.52 |
41d | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|108951132 | 142 | 19% 41% | 7 2 | 51.13/5.91 53.40/6.07 | 13.33/4.99 | 1.00 | 0.94 | 0.00 | +∞ |
CCD31477 | 140 | ||||||||||
43 | Oxygen-evolving enhancer protein 2, chloroplastic | gi|449460024 | 383 | 57% | 15 | 28.12/8.61 | 17.00/6.26 | 1.00 | 0.88 | 0.69 | 0.66 |
44 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 411 | 50% | 19 | 51.46/6.00 | 37.25/6.59 | 1.00 | 0.70 | 0.37 | 1.03 |
48 | Thylakoid lumenal 29 kDa protein, chloroplastic | gi|449436992 | 538 | 49% | 19 | 40.25/7.66 | 30.40/6.94 | 1.00 | 3.44 | 1.32 | 1.22 |
51 | Oxygen-evolving enhancer protein 2, chloroplastic | gi|449460024 | 396 | 61% | 13 | 28.12/8.61 | 11.50/5.23 | 1.00 | 0.89 | 0.69 | 0.46 |
52 | Ribulose bisphosphate carboxylase large chain, partial | gi|659133948 | 358 | 34% | 10 | 30.15/6.23 | 9.75/5.24 | 1.00 | 1.55 | 0.44 | 1.78 |
53 | Ribulose 1,5-bisphosphate carboxylase/oxygenase large Subunit (plastid) | gi|590000423 | 309 | 28% | 14 | 52.61/6.00 | 9.50/5.11 | 1.00 | 0.93 | 0.32 | 1.67 |
54 | Ribulose bisphosphate carboxylase large chain, partial | gi|659133948 | 224 | 35% | 11 | 30.15/6.23 | 9.25/4.99 | 1.00 | 0.94 | 0.43 | 1.26 |
55d | Ribulose-1,5-bisphosphate carboxylase/oxygenase large Subunit, partial (chloroplast) | gi|111182716 CCD31477 | 170 177 | 19% 56% | 10 4 | 52.20/6.00 5.34/6.07 | 10.25/4.68 | 1.00 | 0.82 | 0.26 | 1.69 |
56 | Plastocyanin A, chloroplast | gi|700204935 | 260 | 41% | 4 | 17.01/4.92 | 8.00/4.41 | 1.00 | 1.96 | 0.87 | 0.46 |
60 | Beta-form rubisco activase | gi|700195391 | 568 | 53% | 18 | 48.29/8.19 | 48.25/5.53 | 1.00 | 1.57 | 1.59 | 0.99 |
62 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 497 | 51% | 25 | 51.46/6.00 | 40.75/6.95 | 1.00 | 16.53 | 1.90 | 1.16 |
63 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 753 | 51% | 22 | 51.46/6.00 | 39.25/6.97 | 1.00 | 14.48 | 1.32 | 3.62 |
64 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 676 | 45% | 22 | 51.46/6.00 | 34.00/6.35 | 1.00 | 0.91 | 0.65 | 1.07 |
66 | Ribulose bisphosphate carboxylase small chain, chloroplastic | gi|449434620 | 456 | 57% | 14 | 20.69/8.24 | 8.75/5.73 | 1.00 | 2.68 | 1.31 | 1.27 |
67 | Ribulose bisphosphate carboxylase small chain, chloroplastic | gi|449434620 | 405 | 55% | 13 | 20.69/8.24 | 7.25/5.40 | 1.00 | 0.97 | 0.34 | 1.96 |
70 | Beta-form rubisco activase | gi|700195391 | 723 | 63% | 23 | 48.29/8.19 | 47.25/5.81 | 1.00 | 1.52 | 1.55 | 1.05 |
71 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 756 | 42% | 21 | 51.46/6.00 | 34.50/6.46 | 1.00 | 0.81 | 0.65 | 0.99 |
72 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 586 | 42% | 21 | 51.46/6.00 | 34.25/6.52 | 1.00 | 0.77 | 0.63 | 1.31 |
75 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | gi|111182702 | 502 | 49% | 24 | 51.46/6.00 | 35.00/6.25 | 1.00 | 0.56 | 0.87 | 0.79 |
77 | plastocyanin A, chloroplast | gi|700204935 | 410 | 39% | 3 | 17.01/4.92 | 9.5/4.45 | 1.00 | 0.88 | 0.65 | 0.77 |
Energy and metabolism (14) | |||||||||||
8 | ATP synthase CF1 beta subunit (plastid) | gi|590000422 | 904 | 69% | 28 | 53.81/5.11 | 59.20/5.19 | 1.00 | 1.52 | 1.17 | 1.11 |
9 | ATP synthase subunit beta, mitochondrial-like | gi|449465916 | 1,020 | 73% | 30 | 60.07/5.90 | 58.98/5.34 | 1.00 | 1.17 | 1.34 | 0.51 |
13 | Transketolase, chloroplastic | gi|351735634 | 197 | 45% | 27 | 80.57/6.00 | 96.50/5.75 | 1.00 | 2.18 | 2.00 | 0.69 |
14 | Transketolase, chloroplastic | gi|351735634 | 453 | 54% | 32 | 80.57/6.00 | 95.17/5.81 | 1.00 | 1.67 | 1.40 | 0.61 |
18d | ACT domain-containing protein ACR11 | gi|449439743 XP_022153437 | 221 213 | 35% 21% | 9 8 | 31.96/5.53 31.97/5.71 | 35.00/4.72 | 1.00 | 2.42 | 3.05 | 0.38 |
31 | Fructose-bisphosphate aldolase 1, chloroplastic | gi|449464838 | 455 | 64% | 21 | 42.87/6.38 | 39.50/5.72 | 1.00 | 1.56 | 1.30 | 1.44 |
45 | Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 | gi|449444016 | 708 | 62% | 19 | 43.08/6.19 | 42.00/6.26 | 1.00 | 0.42 | 1.48 | 0.42 |
50 | Nucleoside diphosphate kinase | gi|659074723 | 308 | 42% | 7 | 16.40/6.30 | 8.40/6.41 | 1.00 | 1.06 | 0.56 | 3.22 |
57 | Malate dehydrogenase, mitochondrial | gi|700198438 | 478 | 54% | 13 | 36.18/8.52 | 38.44/6.25 | 1.00 | 0.33 | 0.87 | 0.71 |
58 | Fructose-bisphosphate aldolase 1, chloroplastic | gi|449464838 | 254 | 44% | 13 | 42.87/6.38 | 39.75/5.81 | 1.00 | 1.19 | 2.43 | 0.54 |
59 | Fructose-bisphosphate aldolase 1, chloroplastic | gi|449464838 | 340 | 60% | 19 | 42.87/6.38 | 39.75/5.88 | 1.00 | 0.54 | 1.21 | 0.86 |
61 | S-adenosylmethionine synthase 2 | gi|449472806 | 764 | 80% | 24 | 43.20/5.35 | 53.5/5.61 | 1.00 | 0.48 | 0.54 | 0.69 |
65 | Nucleoside diphosphate kinase | gi|700198251 | 152 | 43% | 9 | 25.96/9.18 | 11.75/6.75 | 1.00 | 1.56 | 1.05 | 1.52 |
73 | Enolase isoform X1 | gi|449451102 | 421 | 58% | 18 | 47.71/5.48 | 62.50/5.72 | 1.00 | 1.48 | 3.20 | 0.54 |
Defense response (11) | |||||||||||
5 | Abscisic stress ripening-like protein | gi|700190659 | 487 | 66% | 19 | 31.72/5.02 | 59.02/4.87 | 1.00 | 2.02 | 0.95 | 0.72 |
6 | Abscisic stress ripening-like protein | gi|700190659 | 621 | 67% | 21 | 31.72/5.02 | 59.11/4.89 | 1.00 | 11.55 | 5.03 | 1.83 |
7 | Abscisic stress ripening-like protein | gi|700190659 | 832 | 72% | 21 | 31.72/5.02 | 58.00/4.93 | 1.00 | 5.86 | 5.43 | 0.62 |
16 | Peroxidase | gi|700198939 | 589 | 52% | 11 | 34.28/4.94 | 40.25/4.78 | 1.00 | 0.62 | 1.11 | 0.40 |
17 | Chromoplast-specific carotenoid-associated protein, chromoplast | gi|449434000 | 682 | 64% | 15 | 35.22/5.05 | 36.75/4.77 | 1.00 | 2.40 | 2.90 | 0.58 |
34d | Ascorbate peroxidase | gi|525507192 AGJ72851 | 662 652 | 71% 72% | 15 14 | 27.38/5.43 27.41/5.43 | 32.50/5.78 | 1.00 | 1.30 | 3.03 | 0.61 |
40d | 2-Cys peroxiredoxin BAS1, chloroplastic | gi|659084460 XP_022144676 | 442 442 | 38% 40% | 10 10 | 30.03/8.32 29.55/7.66 | 20.75/5.04 | 1.00 | 2.30 | 1.44 | 0.96 |
46 | Leghemoglobin reductase | gi|449459772 | 514 | 56% | 23 | 53.57/7.68 | 64.00/6.79 | 1.00 | 1.55 | 3.02 | 0.93 |
49 | Peptide methionine sulfoxide reductase A1-like | gi|778690317 | 333 | 35% | 8 | 29.89/8.96 | 26.25/6.73 | 1.00 | 2.09 | 1.38 | 1.13 |
68 | Catalase isozyme 3 | gi|700192329 | 585 | 52% | 24 | 57.01/6.84 | 59.00/6.94 | 1.00 | 6.25 | 2.07 | 1.13 |
69 | Catalase isozyme 1 | gi|778697155 | 638 | 62% | 25 | 57.05/6.80 | 57.29/6.96 | 1.00 | 4.57 | 2.58 | 1.30 |
Protein and nucleic acid biosynthesis (9) | |||||||||||
3 | Protein disulfide-isomerase | gi|700192511 | 879 | 72% | 35 | 57.05/4.88 | 72.07/4.86 | 1.00 | 1.68 | 2.54 | 0.54 |
12 | Elongation factor G-2, chloroplastic | gi|449459756 | 372 | 46% | 29 | 85.63/5.42 | 108.25/5.34 | 1.00 | 0.32 | 0.50 | 1.19 |
19 | Glycine-rich RNA-binding protein blt801 | gi|778656094 | 484 | 53% | 11 | 28.46/5.07 | 33.75/4.69 | 1.00 | 0.95 | 0.61 | 0.78 |
22 | 30S ribosomal protein S1, chloroplastic | gi|449459770 | 851 | 51% | 21 | 45.30/5.34 | 48.00/5.17 | 1.00 | 0.95 | 1.54 | 0.60 |
24d | Actin-7 | gi|449459238 XP_022132857 | 454 454 | 63% 63% | 18 18 | 41.68/5.31 41.68/5.31 | 49.25/5.34 | 1.00 | 0.98 | 0.61 | 1.24 |
30 | Protease Do-like 1, chloroplastic isoform X1 | gi|449450105 | 739 | 50% | 18 | 46.81/7.13 | 39.50/5.54 | 1.00 | 1.56 | 1.25 | 1.15 |
47 | Serine hydroxymethyltransferase | gi|700193477 | 702 | 61% | 30 | 57.84/8.12 | 61.5/6.80 | 1.00 | 0.89 | 3.09 | 0.97 |
74 | 29 kDa ribonucleoprotein, chloroplastic | gi|449440111 | 173 | 47% | 9 | 30.48/5.84 | 24.33/4.88 | 1.00 | 0.36 | 0.00 | + ∞ |
76 | Glycine dehydrogenase, mitochondrial | gi|449450349 | 656 | 47% | 35 | 113.29/6.62 | 123.25/6.49 | 1.00 | 0.54 | 1.54 | 0.43 |
Molecular chaperone (7) | |||||||||||
1 | Calreticulin | gi|449454026 | 418 | 55% | 25 | 48.36/4.45 | 62.21/4.40 | 1.00 | 2.66 | 5.13 | 0.25 |
2d | HSP70, chloroplast | gi|700206320 XP_022154567 | 784 774 | 51% 50% | 35 34 | 75.35/5.18 75.18/5.26 | 99.01/4.88 | 1.00 | 0.98 | 1.77 | 0.40 |
11 | Luminal-binding protein 5 | gi|659074058 | 776 | 45% | 29 | 73.43/5.10 | 84.00/5.31 | 1.00 | 0.98 | 3.25 | 0.57 |
33 | 20 kDa chaperonin, chloroplastic | gi|449452602 | 611 | 65% | 17 | 26.87/7.85 | 27.50/5.55 | 1.00 | 1.05 | 1.69 | 0.97 |
38 | Small heat shock protein, chloroplastic-like isoform X1 | gi|778675414 | 506 | 51% | 10 | 20.84/5.03 | 18.67/5.13 | 0.00 | + ∞ | + ∞ | 0.11 |
39 | Small heat shock protein, chloroplastic-like isoform X2 | gi|659095978 | 492 | 48% | 11 | 23.82/6.36 | 18.75/5.03 | 1.00 | 3.65 | 4.75 | 0.62 |
42 | Cytosolic class II low molecular weight heat shock protein | gi|700200202 | 347 | 67% | 7 | 17.48/5.54 | 11.50/5.58 | 1.00 | 0.40 | 0.72 | 0.85 |
Unknown protein (3) | |||||||||||
20 | Uncharacterized protein LOC103496938 | gi|449442405 | 534 | 74% | 22 | 35.16/4.79 | 38.75/4.95 | 1.00 | 1.72 | 1.41 | 0.65 |
29 | Uncharacterized protein LOC101217229 | gi|449463844 | 578 | 59% | 17 | 37.81/6.22 | 37.25/5.35 | 1.00 | 1.20 | 1.90 | 0.56 |
32 | Fruit protein pKIWI502 | gi|449434568 | 525 | 53% | 12 | 32.52/6.08 | 33.50/5.05 | 1.00 | 0.96 | 0.38 | 1.57 |
