Fig. 6: Transient expression assays. | Horticulture Research

Fig. 6: Transient expression assays.

From: The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening

Fig. 6

The promoters of CpPME1/2, CpEXP1/2, and CpPG1/2 were cloned into a pGreenII 0800-LUC double-reporter vector a, while CpEBF1, CpMADS1/3, and CpEIL1 were cloned into the pGreenII 62-SK vector as effectors b. The LUC activity was normalized to the REN activity (internal control). c–f Transcriptional activity of CpEBF1 c, CpMADS1/3 d, e, and CpEIL1 f on the cell wall degradation-related gene promoter of CpPME1/2, CpEXP1/2, and CpPG1/2. g–l Transcriptional activity of CpEBF1, CpMADS1/3, CpEIL1, CpEBF1 + CpMADS1/3, and CpEBF1 + CpEIL1 on the promoters of CpPME1/2 g, h, CpEXP1/2 i, j, and CpPG1/2 k, l. At least six transient assay measurements were performed for each assay. The values represent the means ± SE. Asterisks indicate significantly different values (**P < 0.01)

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