Fig. 7: Enzyme activity and subcellular localization analysis of PmBEATs.
From: Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production
a The benzyl alcohol acetyltransferase activity of PmBEAT34, PmBEAT36 and PmBEAT37. The PmBEAT genes were expressed in tobacco leaves by Agrobacterium injection, and the crude protein was extracted for enzyme activity analysis. The data are presented as the mean values of three replicates ± SD. **P < 0.01 (Student’s t-test). Three independent experiments were performed with similar results. b Detection of the expression levels of PmBEAT34, PmBEAT36 and PmBEAT37 in tobacco leaves by sq-RT-PCR. NbTubulin was used as the reference gene. c Confocal images of petal protoplasts expressing PmBEAT proteins containing the GFP tag. The scale bars are 20 μM
