Fig. 4: Paralogous gene mutations mediated by the CRISPR/Cas9 system. | Horticulture Research

Fig. 4: Paralogous gene mutations mediated by the CRISPR/Cas9 system.

From: CRISPR/Cas9-mediated multiple gene editing in Brassica oleracea var. capitata using the endogenous tRNA-processing system

Fig. 4: Paralogous gene mutations mediated by the CRISPR/Cas9 system.

a Site A, B, C, and D sequence alignment between the Bol016089 and BoPDS genes. b Summary of the mutation types and frequencies at sites A–D in the Bol016089 gene. c Sites A, B, C, and D sequence alignment between the BoSLG3 gene (X79431.1) and the BoSRK3 gene. d Summary of the mutation types and frequencies at site D in the BoSLG3 gene. The number of each mutation type was used to estimate the genome editing efficiency. The target sequences are shown in green and the mutations in red

Back to article page