Fig. 2: Partial non-ripening phenotype of NOR CRISPR/Cas9 knockout. | Horticulture Research

Fig. 2: Partial non-ripening phenotype of NOR CRISPR/Cas9 knockout.

From: Diversity and redundancy of the ripening regulatory networks revealed by the fruitENCODE and the new CRISPR/Cas9 CNR and NOR mutants

Fig. 2

a Position of the NOR gRNA target sites (T2 231–209 bp, T1 281–302 bp, T4 363–341 bp, T3 1169–1191 bp). b Sanger sequencing of the CRISPR edited sites in line #11 (four bases of CTCC located in 215–218 bp and one base of A located in the 269 bp were deleted, CACCGGG located in 219–225 bp were substituted to GGTGGGA) and #19 (GAACT which were located in 347–351 bp were deleted). Red letters indicate the gRNA target sites, green letters represent edited sites and blue letters represent the protospacer adjacent motif (PAM). c The partial non-ripening phenotype of CRISPR/Cas9 fruits compared with that of the wild-type, and non-ripening nor mutant

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