Fig. 3: Phenotype of the CNR CRISPR/Cas9 knockout. | Horticulture Research

Fig. 3: Phenotype of the CNR CRISPR/Cas9 knockout.

From: Diversity and redundancy of the ripening regulatory networks revealed by the fruitENCODE and the new CRISPR/Cas9 CNR and NOR mutants

Fig. 3

a Schematic illustration of four gRNA target sites in CNR locus (T1 535–557 bp, T2 564–586 bp, T3 604–582 bp, T4 712–690 bp). b Sanger sequencing of the CRISPR edited sites in line #22 (seven bases of AAGCTAG located in 547–553 bp were deleted, one base of T and one base of G were inserted after 580 bp and 695 bp, respectively) and #23 (eight bases of GAAGCTAG located in 546–553 bp were deleted). Red letters indicate the target sites, green letters represent edited sites, blue letters represent the PAM. c The CNR CRISPR/Cas9 fruits only showed 2–3 days of delayed ripening compared with that of the WT and the fruits obtained the full color finally, while the original cnr mutant failed to ripen

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