Fig. 1: Pattern of eGFP expression and protein accumulation among individual fruits.

a RT-qPCR analysis of eGFP expression, showing variation in characteristics among individual fruits. Detached fruits with a specimen number of 30 were individually infected with Agrobacterium carrying the eGFP reporter gene, and gene expression was assessed 4 days after infection. The horizontal ordinate represents relative gene expression, and the whole value range was divided into successive subranges with a span of 0.4. The vertical ordinate represents the number of fruits with the values of gene expression within each subrange. FaACTIN was used as a normalized control gene. b eGFP protein images showing a variation in pattern of eGFP protein accumulation. Twelve representative fruits are shown. EV empty vector