Fig. 4: Effect of temperature on transient gene manipulation. | Horticulture Research

Fig. 4: Effect of temperature on transient gene manipulation.

From: Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits

Fig. 4

Detached LG fruits were infected with Agrobacterium carrying the eGFP reporter gene driven by the 35CaMV promoter. After infection, fruits were incubated at different temperatures at 100% humidity. eGFP image assays were conducted on the 5th day after Agrobacterium infection. a eGFP images. b qRT-PCR analysis of eGFP gene expression. FaACTIN was used as a normalized control gene. Values are means ± SD of six fruits. Statistically significant differences among samples were tested by Tukey’s test, and significant differences at the P < 0.05 level are indicated by different letters

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