Table 2 Mutations identified in the pomegranate ugt84a23 ugt84a24 hairy root lines.
sgRNA | Mutations in the target gene alleles | |
|---|---|---|
PgUGT84A23 | PgUGT84A24 | |
23+24-1 (305) | WT, 6-bp deletion (GGCACG), 7-bp deletion (CCGGGTC), 13-bp insertion (TGAATTAGTTAGT) | 1-bp deletion (C), 30-bp deletion (CGTCGCCTATCGGGGACGGGTTCATTAGGT), 1162-bp deletion (CCTCGATCAGTACTTGCCCCAGCTCGAGAAAGTCGGCAAGGAGGTACATATAACTATATATATTATAAATCAGTTAGGAGTTAATTAATTATATGAGTTCTTCGTAATTTTTTTCTCCTGGAATTTTAATTAAAAAAAAGTTAGGAGTTGCTAAATTATTTGGATGCAGACAGACTTCAAATTATGAAAACGGTTTTGGTTTGGTCCATATAACAAAATCATTTATAGTACGCAATTCTACTCTACACATTTTTCAGCTTATACCCCATTAATTGGAGAGTAATTTCTTCATATTCCTACATTCAGGTTGGCAAAATTTTTAATATACCTTTTCGGTTATAAAATTTTTAATATACCTTAGCCGAGGACAACTATATTCATCTTAAACTACCCAAAATATATGCCGATTCAAATGCAGGAATTTGAAGGTATAATATTAAATACTTGGTCCAGACAAGCCATAATAATTAAATAAGGAATCTATATATGGTTTGCAATATTAGGACATAAATAATAAAACATGCCATCATTTTACAGTATTAACTTTTTCTAGAGCACGTATAGTTTTTCAACTTTTTCCTTTTGGGTGAACAGAGCACAATATAGTTATGATAATACATTGAAAACTAAGTCTAAAATACGAGAAATGATTTGGTAATATTTTTTTTTGTGCAAACACTTGTATTCGGAAGCCTAATTGGATCTTAACTAATTCAGTTGAACCGAGTCGATTCACTAAGGGGGTAAAACTCTCATAATAATATTACCTTTATCATGTTATGTGAGAAAACACTAATTAGATTACAACAGGTAAATGAATGCTAATCGTTCGAATAAATATCATTTGTTCTTAATTAAGGTAATATTATTGATTTTTCCTCGTCAGATCATTGTTGGGGAAGTACAATCCTCACCTATAACTTATTATTTTCCAGAGTTGGTGACAAAATTACGACATTGAAATTGATGAAGGGAATACGATATTGATTTTGTCTTTCGATTCATTCACAAATGAAGTGCGATCAATAATGTCTATCACGGACACTGCAGGTAATTCCACGGATGATAAAGAAGAACGAGGAGCAGAACCGTCCCGTGTCCTGCCTCATCAACAACCCGTTCATCCCGTGGGTGTCTGACGTGGCCGAGAGTCTCGGTCTAC) |
23+24-1 (307) | Homozygous 2-bp deletion (TC) | 3-bp deletion (GCC), 19-bp deletion (CGGGGAGGAGCCGTCGCCT) |
23+24-1 (310) | Homozygous 10-bp deletion (GTCAAGGGCA) | Homozygous 1-bp deletion (C) |
23+24-1 (316) | Homozygous 1-bp deletion (G) | 1-bp deletion (C), 3-bp deletion (CGC) |
23+24-1 (318) | 1-bp mismatch (G/T), 3-bp deletion (TCA), 9-bp deletion (GTCAAGGGC), 20-bp deletion (CTTGGTCTCCTTCCCGGGTC), 25-bp deletion (TTCTTGGTCTCCTTCCCGGGTCAAG) | WT, 18-bp deletion (GAGCCGTCGCCTATCGGG) |
23+24-1 (319) | Homozygous 1-bp deletion (T) | 1-bp deletion (T), 29-bp deletion (CGTCGCCTATCGGGGACGGGTTCATTAGG) |
23+24-1 (321) | WT, [21-bp mismatch and 6-bp deletion (GAAGGGTTCACCCGACCACAC in mutant vs. TTCCCGGGTCAAGGGCACGTGAACCCA in wild type)] | 1-bp deletion (T), 8-bp deletion (CGTCGCCT) |
23+24-1 (322) | Homozygous 2-bp insertion (GC) | 6-bp deletion (GTCGCC), 7-bp insertion (TCCTTTT) |
23+24-1 (323) | Homozygous 1-bp deletion (G) | 3-bp deletion (CGC), 21-bp deletion (GAGGAGCCGTCGCCTATCGGG) |
23+24-1 (324) | 1-bp deletion (G), 3-bp deletion (GGG) | 1-bp deletion (T), 6-bp deletion (TCGCCT) |
23+24-1 (325) | 3-bp deletion (TCA), 4-bp deletion (TCAA) | 6-bp deletion (GCCGTC), 7-bp deletion (TCGCCTA) |
23+24-1 (327) | 1-bp insertion (T), 5-bp deletion (GGGTC) | 1-bp insertion (A), 3-bp deletion (CGC) |
23+24-1 (346) | 4-bp deletion (TCAA), 7-bp deletion (CCGGGTC) | 21-bp deletion (GAGGAGCCGTCGCCTATCGGG), 68-bp deletion (CGCCTATCGGGGACGGGTTCATTAGGTTCGAGTTCTTTGAAGATGGATGGGACGAGGATGAGCCCCGG) |
23+24-2 (309) | 1-bp mismatch (A/T), 9-bp deletion (TCAAGGGCA) | 2-bp deletion (TG), 59-bp deletion (CAACAACCCGTTCATCCCGTGGGTGTCTGACGTGGCCGAGAGTCTCGGTCTACCCTCGG) |
23+24-2 (312) | 1-bp mismatch (C/T), 12-bp deletion (CGGGTCAAGGGC) | WT, 1-bp insertion (T) |
23+24-2 (313) | WT, 1-bp deletion (G), 5-bp deletion (GTCAA), 124-bp deletion (TCTCGAATTATTAGCCGCAGAAGAAGAAGCAGAAGAAGGAATTACAGGTGAATTAGTTAGTTCATTATGGGTTCGGAGTCGTCACTTGTCCATGTCTTCTTGGTCTCCTTCCCGGGTCAAGGGC) | 5-bp deletion (GTCTG), 7-bp deletion (GTCTGAC) |
23+24-2 (328) | 1-bp deletion (G), 3-bp deletion (TCA) | 1-bp insertion (T), 6-bp deletion (TGACGT) |
23+24-2 (333) | WT, 3-bp deletion (GTC), 10-bp deletion (GGGTCAAGGG) | WT, 1-bp insertion (A), 1-bp insertion (T) |
23+24-2 (335) | 3-bp deletion (GTC), 35-bp deletion (TCACTTGTCCATGTCTTCTTGGTCTCCTTCCCGGG) | 1-bp insertion (A), 1-bp deletion (G) |
