Fig. 4: Semi-RT-PCR analysis of 67 MFS-type sugar transporter (ST) genes in F. × ananassa cv. Benihoppe.

The number of repeat cycles for STs is 35, and that for the internal control, FaCHP1, is 28. Fruits in four stages (G-green, W- white, T- turning; R-ripe) were dissected into achenes (A) and flesh (F). L, The third fully expanded leaf blades were simultaneously sampled from fruiting plants. Two biological replicates (from two independent RNA samples) of each tissue were included in the analysis, and for each biological replicate, three technical replicates were performed