Fig. 1: In vitro culture system for studying adventitious root formation, symbiosis, and plant growth. | Horticulture Research

Fig. 1: In vitro culture system for studying adventitious root formation, symbiosis, and plant growth.

From: Ericoid mycorrhizal fungus enhances microcutting rooting of Rhododendron fortunei and subsequent growth

Fig. 1

a Microcuttings (the insertion on the upper left corner) derived from in vitro cultured R. fortunei grown in a sterilized peat-based substrate without (CK) and with inoculation of Om19 (Om19). b AR formation and Om19 colonization: Mycelium (sky blue arrow) of Om19 appeared on the surface of an AR formed from the base of a cutting in one weeks (a), mycelium coils (sky blue arrow) occurred in rhizodermal and cortex cells of ARs in two-three weeks (b), rhizodermal and cortex cells of lateral roots (black arrow) were colonized by Om19 with dense coin in four weeks (c), and no mycelium on root surface or rhizodermal cells of ARs formed from cuttings without Om19 inoculation (d). c The growth of ARs and shoot leaf numbers: AR number (a), average root length (b), and leaf numbers (c) of microcuttings produced during a 120-day growth period in the sterilized peat-based substrate uninoculated (CK) and inoculated (Om19) with Om19. Bars represent standard errors (n = 5) where (*) and (**) indicate significant differences in a given parameter between CK and Om19 treatments in the sampling date based on Tukey’s HSD test at P < 0.05 and P < 0.01 levels, respectively

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