Fig. 4: Characterization of pollen phenotypes in SlMYB33-RNAi lines.
From: The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato
a, d In vitro pollen germination tests in WT (a) and SlMYB33-RNAi line 10 (d). Bars = 100 μm. b, e Scanning electron microscopy (SEM) images of pollen grains in WT (b) and RNAi-10 (e). Bars = 25 μm. c, f Single pollen grain morphology under SEM in WT (c) and RNAi-10 (f). Bars = 5 μm. g, h Quantification of the pollen germination rate (g) and percentage of morphologically normal pollen grains (h) in WT and different RNAi lines. Values are the means ± SD of ten biological replicates from three plants, and three to four flowers were examined in each plant. Asterisks indicate significant differences between the RNAi lines and the WT by Student’s t test (**P < 0.01)
