Fig. 1: MdBT2 interacts with MdMYB73. | Horticulture Research

Fig. 1: MdBT2 interacts with MdMYB73.

From: BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

Fig. 1

a, b The interaction between MdBT2 and MdMYB73 in a Y2H assay. The full-length cDNA and five truncated mutants of the MdBT2 gene (a) were inserted into the pGBT9 vector. The full-length cDNA of MdMYB73 (a) was inserted into the pGAD424 vector. Panels show the different interactions, as indicated by yeast growth (a). The full-length cDNA and four truncated mutants of the MdMYB73 gene (b) were inserted into the pGAD424 vector. The full-length cDNA of the MdBT2 gene (b) was inserted into the pGBT9 vector. Panels show the interactions as indicated by yeast growth (b). c MdBT2 interacted with MdMYB73, as indicated by pull-down assay. MdBT2-GST expression in E. coli resulted in the precipitation of MdMYB73-HIS, with the anti-GST antibody and anti-HIS antibody. GST alone was used as the control. d MdBT2 interacted with MdMYB73 in a BiFC assay using tobacco leaf cells. MdBT2 + N and MdMYB73 + C interacted in the nucleus of tobacco leaf cells. Scale bar = 20 µm. The data were obtained from at least two biological replicates

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