Fig. 2: MdBT2 affects the stability of the MdMYB73 protein. | Horticulture Research

Fig. 2: MdBT2 affects the stability of the MdMYB73 protein.

From: BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

Fig. 2

a MdBT2 degraded MdMYB73 in vitro. Cell-free degradation assays were performed to examine the stability of the MdMYB73 protein. Three types of apple calli (WT, 35S::MdBT2, and 35S::anti-MdBT2) were coincubated with MdMYB73-HIS protein at 22 °C for the times indicated. b Degradation curve analysis of MdMYB73-HIS. c The stability of the MdMYB73 protein after MG132 treatment. WT, 35S::MdBT2, and 35S::anti-MdBT2 transgenic apple calli were treated with 50 mM MG132, and then incubated with MdMYB73-HIS at 22 °C for the times indicated. d Degradation curve analysis of MdMYB73-HIS upon MG132 treatment. The experiments were performed for three biological replicates, and the data are presented as the means ± standard deviation. Bars with different letters indicate significant differences at P < 0.05

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