Fig. 5: MdMYB73 is required for MdBT2 to control malate accumulation and vacuolar pH in apple calli. | Horticulture Research

Fig. 5: MdMYB73 is required for MdBT2 to control malate accumulation and vacuolar pH in apple calli.

From: BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

Fig. 5

a Expression analysis of malate-related genes, MdVHA-A, MdVHP1, and MdALMT9, using qRT-PCR in WT and transgenic apple calli (35S::anti-MdMYB73, 35S::anti-MdBT2, and 35S::anti-MdBT2 + 35S::anti-MdMYB73). b The malate concentration in the WT and transgenic apple calli (35S::anti-MdMYB73, 35S::anti-MdBT2, and 35S::anti-MdBT2 + 35S::anti-MdMYB73). c–e Hydrolytic (c) and proton-pumping activity levels of V-ATPase (d) and V-PPase (e) in WT and transgenic apple calli (35S::anti-MdMYB73, 35S::anti-MdBT2, and 35S::anti-MdBT2 + 35S::anti-MdMYB73). f, g Emission intensities (f) and quantitative analysis (g) of protoplasts to determine the vacuolar pH in apple calli with BCECF dyes at 488 nm and 458 nm (WT, 35S::anti-MdBT2, 35S::anti-MdMYB73, and 35S::anti-MdBT2 + 35S::anti-MdMYB73). Scale bar = 10 µm. Bars with different letters are significantly different at P < 0.05, according to Tukey’s single factor tests. Data are shown as the means ± SD

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