Table 5 Summary of the variants identified in the four S. pimpinellifolium and four S. lycopersicum var. cerasiforme accessions using using Heinz 1706 SL4.0 as a reference genome26 (RF).
S. pimpinellifolium | S. lycopersicum var. cerasiforme | Total | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SP1 | SP2 | SP3 | SP4 | Mean | SLC1 | SLC2 | SLC3 | SLC4 | Mean | |||||||||||||
Count | % | Count | % | Count | % | Count | % | Mean | % | Count | % | Count | % | Count | % | Count | % | Count | % | Count | % | |
Variants | ||||||||||||||||||||||
Homozygous SNPs | 3,223,730 | 69.5 | 3,032,979 | 78.5 | 3,879,974 | 79.4 | 2,453,673 | 76.9 | 3,147,589 | 76.1 | 1,497,288 | 76.0 | 855,877 | 68.9 | 912,198 | 69.3 | 1,115,582 | 72.2 | 1,095,236 | 71.6 | 9,106,964 | 86.2 |
Heterozygous SNPs | 647,201 | 14.0 | 184,801 | 4.8 | 209,810 | 4.3 | 189,876 | 6.0 | 307,922 | 7.3 | 129,834 | 6.6 | 125,920 | 10.1 | 143,599 | 10.9 | 149,794 | 9.7 | 137,287 | 9.3 | ||
Homozygous InDels | 379,109 | 8.2 | 381,565 | 9.9 | 447,678 | 9.2 | 324,948 | 10.2 | 383,325 | 9.4 | 217,208 | 11.1 | 172,779 | 13.9 | 161,126 | 12.2 | 175,312 | 11.3 | 181,606 | 12.1 | 812,034 | 7.7 |
Heterozygous InDels | 84,534 | 1.8 | 18,288 | 0.4 | 20,876 | 0.4 | 16,667 | 0.5 | 35,091 | 0.8 | 11,262 | 0.6 | 9,928 | 0.8 | 9,689 | 0.7 | 10,972 | 0.7 | 10,463 | 0.7 | ||
Homozygous complex | 209,751 | 4.5 | 188,390 | 4.8 | 258,072 | 5.3 | 151,032 | 4.8 | 201,811 | 4.9 | 76,890 | 3.9 | 53,003 | 4.2 | 60,497 | 4.6 | 61,577 | 4.0 | 62,992 | 4.2 | 579,456 | 5.5 |
Heterozygous complex | 57,559 | 1.2 | 26,997 | 0.7 | 32,071 | 0.6 | 24,840 | 0.8 | 35,367 | 0.8 | 18,838 | 0.9 | 15,018 | 1.2 | 18,388 | 1.4 | 18,824 | 1.2 | 17,767 | 1.2 | ||
Homozygous MNP | 32,803 | 0.7 | 29,863 | 0.8 | 36,797 | 0.7 | 23,648 | 0.7 | 30,778 | 0.7 | 15,916 | 0.8 | 9,748 | 0.8 | 11,157 | 0.8 | 12,513 | 0.8 | 12,334 | 0.8 | 58,804 | 0.6 |
Heterozygous MNP | 4906 | 0.1 | 2144 | 0.1 | 2469 | 0.1 | 1937 | 0.1 | 2864 | 0.1 | 1501 | 0.1 | 1,055 | 0.1 | 1,261 | 0.1 | 1,355 | 0.1 | 1,293 | 0.1 | ||
Total homozygous | 3,845,393 | 82.9 | 3,632,797 | 94.0 | 4,622,521 | 94.6 | 2,953,301 | 92.7 | 3,763,503 | 91.1 | 1,807,302 | 91.8 | 1,091,407 | 87.8 | 1,144,978 | 86.9 | 1,364,984 | 88.3 | 1,352,168 | 88.7 | – | – |
Total heterozygous | 794,200 | 17.1 | 232,230 | 6.0 | 265,226 | 5.4 | 233,320 | 7.3 | 381,244 | 9.0 | 161,435 | 8.2 | 151,921 | 12.2 | 172,937 | 13.1 | 180,945 | 11.7 | 166,810 | 11.3 | – | – |
Total variants | 4,639,593 | 100.0 | 3,865,027 | 100 | 4,887,747 | 100.0 | 3,186,621 | 100.0 | 4,144,747 | 100.0 | 1,968,737 | 100.0 | 1,243,328 | 100.0 | 1,317,915 | 100.0 | 1,545,929 | 100.0 | 1,518,977 | 100.0 | 10,557,258 | 100.0 |
% het. variants with RF | 0.103 | 0.030 | 0.034 | 0.030 | 0.049 | 0.021 | 0.020 | 0.022 | 0.023 | 0.022 | ||||||||||||
% total variants with RF | 0.600 | 0.500 | 0.632 | 0.412 | 0.536 | 0.255 | 0.161 | 0.171 | 0.200 | 0.197 | ||||||||||||
Private variants | 771,181 | 16.6 | 748,354 | 19.3 | 1,115,616 | 22.8 | 985,453 | 30.9 | 905,151 | 22.4 | 339,481 | 17.2 | 467,864 | 37.6 | 190,559 | 14.5 | 279,295 | 18.0 | 319,299.8 | 21.8 | 4,897,803 | 46.3 |
Variants in coding regions | ||||||||||||||||||||||
Homozygous SNPs | 307,824 | 58.6 | 321,285 | 71.8 | 375,090 | 72.3 | 253,723 | 69.1 | 314,481 | 68.0 | 124,716 | 65.0 | 92,841 | 60.0 | 78,951 | 56.0 | 80,019 | 55.2 | 94,132 | 59.1 | 996,772 | 82.4 |
Heterozygous SNPs | 107,212 | 20.4 | 30,776 | 6.9 | 32,981 | 6.4 | 34,522 | 9.4 | 51,373 | 10.8 | 22,275 | 11.6 | 23,504 | 15.2 | 27,654 | 19.6 | 30,294 | 20.9 | 25,932 | 16.8 | ||
Homozygous InDels | 58,463 | 11.1 | 61,945 | 13.9 | 70,754 | 13.7 | 51,374 | 14.0 | 60,634 | 13.2 | 29,821 | 15.6 | 26,057 | 16.9 | 22,305 | 15.8 | 22,568 | 15.5 | 25,188 | 15.9 | 131,056 | 10.8 |
Heterozygous InDels | 16,675 | 3.2 | 3073 | 0.7 | 3411 | 0.6 | 2899 | 0.8 | 6515 | 1.3 | 1802 | 0.9 | 1,719 | 1.1 | 1,612 | 1.1 | 1,880 | 1.3 | 1,753 | 1.1 | ||
Homozygous complex | 22,053 | 4.2 | 22,772 | 5.0 | 27,306 | 5.3 | 17,461 | 4.8 | 22,398 | 4.8 | 8323 | 4.3 | 6,548 | 4.2 | 5,826 | 4.1 | 5,476 | 3.8 | 6,543 | 4.1 | 76,511 | 6.3 |
Heterozygous complex | 10,199 | 1.9 | 4550 | 1.0 | 5509 | 1.0 | 4551 | 1.2 | 6202 | 1.3 | 3326 | 1.7 | 2,864 | 1.9 | 3,763 | 2.7 | 3,835 | 2.6 | 3,447 | 2.2 | ||
Homozygous MNP | 2831 | 0.5 | 2816 | 0.6 | 3228 | 0.6 | 2206 | 0.6 | 2770 | 0.6 | 1282 | 0.7 | 947 | 0.6 | 849 | 0.6 | 872 | 0.6 | 988 | 0.6 | 5,909 | 0.5 |
Heterozygous MNP | 749 | 0.1 | 327 | 0.1 | 342 | 0.1 | 315 | 0.1 | 433 | 0.1 | 220 | 0.1 | 170 | 0.1 | 212 | 0.1 | 203 | 0.1 | 201 | 0.1 | ||
Total homozygous | 391,171 | 74.3 | 408,818 | 91.3 | 476,378 | 91.8 | 324,764 | 88.5 | 400,283 | 86.5 | 164,142 | 85.6 | 126,393 | 81.7 | 107,931 | 76.4 | 108,935 | 75.0 | 126,850 | 79.7 | – | – |
Total heterozygous | 134,835 | 25.7 | 38,726 | 8.7 | 42,243 | 8.2 | 42,287 | 11.5 | 64,523 | 13.5 | 27,623 | 14.4 | 28,257 | 18.3 | 33,241 | 23.6 | 36,212 | 25.0 | 31,333 | 20.3 | – | – |
Total variants | 526,006 | 100.0 | 447,544 | 100.0 | 518,621 | 100.0 | 367,051 | 100.0 | 464,806 | 100.0 | 191,765 | 100.0 | 154,650 | 100.0 | 141,172 | 100.0 | 145,147 | 100.0 | 158,184 | 100.0 | 1,210,248 | 100.0 |
% het. variants with RF | 0.017 | 0.005 | 0.005 | 0.005 | 0.008 | 0.003 | 0.004 | 0.004 | 0.005 | 0.004 | ||||||||||||
% variants with RF | 0.068 | 0.058 | 0.067 | 0.047 | 0.060 | 0.024 | 0.020 | 0.018 | 0.019 | 0.020 | ||||||||||||