Fig. 1: Characterization of the cucumber WOX1 gene (CsWOX1) in leaf and petal tissues.

a Leaves from the mutant line (mango fruit, mf) and its corresponding WT line (AM218). Six successive leaves (from the right) were harvested from the top of the same plant. b Male flowers of the mf mutant (top) and AM218 (bottom). c The ratio of average leaf width to length in six successive top leaves from the same plant representing different growth stages. Each point represents a value in the set of data, and the short line represents the mean of this set of data. Five biological repeats were included per measurement. d Longitudinal section of a fully expanded second true leaf blade from mf and AM218. Black and green arrows indicate palisade mesophyll and spongy mesophyll cells, respectively. e Scanning electron microscopy images of a mature leaf adaxial surface in the middle of the lamina from mf and AM218. f mRNA in situ hybridization analysis of CsWOX1 expression in developing leaves of AM218. CsWOX1 transcripts were detected at the early stage of leaf primordia (f1 and f2), the leaf margins (f3, black arrows), and the middle mesophyll layer (f4, black arrows). Sense probe analyses of the leaf (f5) as a negative control. Bar = 5 cm (a), 1 cm (b), 100 µm (d), and 20 µm (e, f)