Fig. 2: Complementation assay of CsWOX1 in Arabidopsis wox1 prs double mutants.

a Aerial view of three different Arabidopsis seedlings. The Arabidopsis wox1 prs (Atwox1 prs) double mutant was obtained by the CRISPR-Cas9 method, and overexpression of CsWOX1 in this double mutant produced the 35S:CsWOX1 Atwox1 prs lines. The narrower leaves of the wox1 prs double mutant were rescued by overexpression of CsWOX1. b Flowers from the WT, 35S:CsWOX1 Atwox1 prs line, and wox1 prs double mutant. c Individual petals from the flowers shown in (b). The narrower petals of the wox1 prs double mutant were also rescued by overexpression of CsWOX1. d Measurements of the leaf (top) and petal (bottom) width in the WT, 35S:CsWOX1 Atwox1 prs line, and wox1 prs double mutant. The sixth fully expanded rosette and flowers in the top inflorescence of the main stem were used to measure the width. Each point represents a value in from the dataset, and the short black line represents the mean of this set of data. Ten biological repeats were included per measurement. Significance tests were performed using Student’s t-test at the 0.05 and 0.01 levels (*p-value <0.05; **p-value <0.01). Arabidopsis Columbia ecotype (Col) was used as the wild type (WT) in all assays. Bar = 1 cm (a) and 1 mm (b, c)