Fig. 7: SlNFYA10 binding to the SlGGP1 promoter. | Horticulture Research

Fig. 7: SlNFYA10 binding to the SlGGP1 promoter.

From: A CCAAT-binding factor, SlNFYA10, negatively regulates ascorbate accumulation by modulating the d-mannose/l-galactose pathway in tomato

Fig. 7: SlNFYA10 binding to the SlGGP1 promoter.

a Analysis of CCAAT elements in the 1.5 kb SlGGP1 promoter region. b Interaction between the SlGGP1 promoter and SlNFYA10 in a yeast one-hybrid assay. The SlGGP1 promoter was inserted into a pAbAi vector as bait, and SlNFYA10 was inserted into pGADT7 as prey. Cotransformed yeast cells were grown on selective media with or without 10 ng/ml AbA. c Transient expression in tobacco leaves. The SlGGP1 promoter was ligated into pGreenII 0800-LUC as a reporter vector, and SlNFYA10 was ligated into pGreenII 62-SK as an effector vector. PG, pGreenII 62-SK empty vector

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